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International Institute of Fisheries Economics and Trade U.S. National Marine Fisheries Service MG Kailis Group
Objectives: The study sets to answer questions regarding factors determining women recruitment into local councils’ leadership positions. It was also intended to establish political leadership selection opportunities for urban and rural women councillors, quota versus non-quota women councillors and men versus women councillors.
Tryptic digestion is an important preanalytical step in shotgun proteomics because inadequate or excessive digestion can result in a failed or incomplete experiment. Unfortunately, this step is not routinely monitored before mass spectrometry because methods available for protein digestion monitoring either are time/sample consuming or require expensive equipment. To determine if a colorimetric method (ProDM Kit) can be used to identify the extent of tryptic digestion that yields the best proteomics outcome, plasma and serum digested for 8 h and 24 h were screened with ProDM, Bioanalyzer, and LC/MS/MS, and the effect of digestion on the number of proteins identified and sequence coverage was compared. About 6% and 16% less proteins were identified when >50% of proteins were digested in plasma and serum, respectively, compared to when ~...
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