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The cryostats of the ATLAS LAr calorimeter system are installed in the ATLAS cavern since several years. Following this, an effort to install and commission the front end read-out electronics (infrastructure, crates, boards) has been ongoing and is finished now, in time for the cavern closure. Following cautious procedures and with continuous testing-campaigns of the electronics at each step of the installation advancement, the result is a fully commissioned calorimeter with its readout and a small number of non-functional channels. A total of only 0.017% of the read out channels is dead and 0.4% need special treatment for calibration. The presentation will give a general overview of the installation and refurbishment campaign of the ATLAS LAr calorimeter electronics and show results of the calibration runs that were taken continuously...
orange pericarp (orp) is a seedling lethal mutant of maize caused by mutations in the duplicate unlinked recessive loci orp1 and orp2. Mutant seedlings accumulate two tryptophan precursors, anthranilate and indole, suggesting a block in tryptophan biosynthesis. Results from feeding studies and enzyme assays indicate that the orp mutant is defective in tryptophan synthase beta activity. Thus, orp is one of only a few amino acid auxotrophic mutants to be characterized in plants. Two genes encoding tryptophan synthase beta were isolated from maize and sequenced. Both genes encode polypeptides with high homology to tryptophan synthase beta enzymes from other organisms. The cloned genes were mapped by restriction fragment length polymorphism analysis to approximately the same chromosomal locations as the genetically mapped factors orp1 and ...
We describe the first virus-like particle of a hyperthermophilic euryarchaeote which was discovered in a strain of “Pyrococcus abyssi” previously characterized in our laboratory. This particle, named PAV1, is lemon-shaped (120 nm × 80 nm), with a short tail terminated by fibers, and resembles the virus SSV1, the type member of the Fuselloviridae, isolated from Sulfolobus shibatae. Sensitivity of the virus-like particle to organic solvents and detergents suggested that the envelope of PAV1 may contain lipids in addition to proteins. It contains a double-stranded circular DNA of 18 kb which is also present in high copy number in a free form in the host cytoplasm. No integrated form of the PAV1 genome could be detected in the host chromosome. Under standard growth conditions, the host cells continuously release PAV1 particles into the cul...
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