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A holographic recording method using an optical heterodyne 2-D scanning technique

for 3-D imaging of fluorescent objects in turbid media is described and experimentally

demonstrated. For the first time, 3-D imaging of fluorescentobjects in turbid media by a

holographic method is achieved, and the diffused photon rejecting process through a

heterodyne technique is analyzed. We also propose and realize a multiplexing and a digital

decoding method for removing twin-image noise in optical scanning holography. The

holographic method studied can be applied to 3-D biomedical imaging of fluorescent

objects in turbid media as well as diffusely reflecting objects.

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